Mice: Breeding and Care

All animal experiments were performed under the approval of the Institutional Animal Care and Use Committee of the University of Texas MD Anderson Cancer Center. C57BL/6J mice (C57BL6, stock # 000664); B6.129P2(C)-Cd19tm1(cre)Cgn/J (CD19-Cre mice; stock #006785), B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (Ai14 mice; stock #007908), B6.Cg-Tg(Col1a1^*2.3-GFP) 1Rowe/J mice (62) (2.3ColGFP mutant mouse strain; stock #013134) were obtained from the Jackson Laboratory. All mice were bred and housed in a specific pathogen-free animal facility at the University of Texas MD Anderson Cancer Center. Ai14 mice were mated to CD19-Cre mice to generate CD19-Cre/ Ai14 mice. Genotyping was performed by PCR using genomic DNA extracted from mouse tails according to Jackson laboratory protocols. 30% of B-cells (B220 positive) express tdTomato in CD19-Cre/ Ai14 mice (63). Ai14/CD19cre mouse bone marrow was used for experiments.