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MiRNA target prediction

RK Ryo Kimura
VS Vivek Swarup
KT Kiyotaka Tomiwa
MG Michael J. Gandal
NP Neelroop N. Parikshak
YF Yasuko Funabiki
MN Masatoshi Nakata
TA Tomonari Awaya
TK Takeo Kato
KI Kei Iida
SO Shin Okazaki
KM Kanae Matsushima
TK Toshihiro Kato
TM Toshiya Murai
TH Toshio Heike
DG Daniel H. Geschwind
MH Masatoshi Hagiwara
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Potential targets of the DE miRNAs were predicted using TargetScan 7.1 (Agarwal, Bell, Nam, & Bartel, 2015), an algorithm included in the Ingenuity Pathway Analysis (IPA) miRNA target filter (QIAGEN). To select target genes, we used the following steps. First, we searched the targets for those with a TargetScan total context score of −0.2 or less. We then selected target genes that were negatively correlated with the DE genes (FDR <0.05) between the WS patients and controls. The remaining gene list was used for further analysis as targets.

Copyright and License information:  ©2018 The Authors. Journal of Child Psychology and Psychiatry published by John Wiley & Sons Ltd on behalf of Association for Child and Adolescent Mental Health.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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