2.5. Measurement of Aβ and NP Uptake by Flow Cytometry

Flow cytometry measurements were performed with the use of the BD-LSR Fortessa (BD Biosciences, San Jose, CA, USA) flow cytometer equipped with BD FACSDiva 8.0.1 software (BD Biosciences, San Jose, CA, USA). Microglial cells were grown overnight in 6-well plates to a density of ca. 1.35 × 10⁵ cells/well. For examination of the Aβ and NP uptake, the cells were treated with Aβ (0.1 μM) and/or CdTeQD (0.1 or 10 μg mL⁻¹) or AgNP (5 or 50 μg mL⁻¹) for 2 h. Then, they were harvested by trypsinization, washed with the growth medium containing 10% FBS and resuspended in the medium containing 2% FBS. Aβ and CdTeQD uptake was estimated on the basis of their mean fluorescence intensity (at 528 and 655 nm, respectively), while AgNP accumulation in the cells was measured as an increase in the mean side scatter (SSC) value [24].