At 4 weeks after implantation, serum was collected from WT mice and GTKO mice. The total IgG and IgM antibodies in WT and GTKO mice serum were measured by ELISA kits (IgG, EMC116, Neobioscience, China; IgM, 88-50470-22, affymetrix Thermo Fisher scientific, USA), following the manufacturer’s instructions.
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