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Cell culture with N-acetylcysteine
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Caco-2 cells were cultured for 15 days in 12-well Transwell inserts as described above. The cells were incubated with the antioxidant N-acetylcysteine (NAC; 4mM) for one hour. Thereafter, they were exposed to 2mg/mL A. simplex CE in the presence of 4mM NAC, and TEER was measured at 1, 4, and 24 hours. Three experiments in triplicates were performed.
Copyright and License information: ©2020 Carballeda-Sangiao et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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