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A total of 3,000 tumor cells/well were seeded in 96-well plates. Tumor cells were subsequently exposed to 100 and 200 µg/ml vitamin C the following day. At 0, 24, 48 and 72 h, MTT reagent (Sigma-Aldrich; Merck Millipore) was added to each well and incubated for 4 h at 37°C. Cell supernatant was subsequently removed and formazan precipitates were dissolved in 150 µl dimethyl sulfoxide. Optical density 570 nm was detected and a growth curve was plotted.
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