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Crystal violet cell proliferation assay

BZ Beibei Zhai
YL Yue Li
SK Sudha Sravanti Kotapalli
JB Jeffrey Bacha
DB Dennis Brown
AS Anne Steinø
MD Mads Daugaard
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Cells were seeded in 96-well culture plates and incubated at 37 °C overnight. Then the cells were treated with different concentrations of DAG for 72 h followed by fixation in 1% glutaraldehyde solution for 5 min. After the fixation, the cells were washed three times with distilled water and stained with 0.1% crystal violet solution for 10 min. Then, the cells were gently rinsed with distilled water to remove non-stained crystal violet dye from the culture plates. After air drying, the remaining crystal violet dye on the plates was dissolved completely in Sorenson’s solution and absorbance at 560 nm wavelength was measured on a BioTek Gen5 microplate reader. The percentages of survival cells after treatment were determined compared with untreated cells.

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