In Vivo Bioluminescence Imaging of Xenograft Model

Bioluminescence imaging was acquired using the IVIS Lumina LT In Vivo Imaging System (Perkin Elmer, Boston, MA, USA) with Living Image version 4.5.5 software (Perkin Elmer, Boston, MA, USA). Mice were infused by intraperitoneal injection with 150 mg/kg D-luciferin potassium salt (Wako, Osaka, Japan) suspended in 200 μL PBS. 5–15 minutes later, mice were imaged under 2% isoflurane anesthesia. Images were acquired on a 25-cm field of view at medium binning level for within 1 min exposure time.