4.6. Mouse Xenograft and In Vivo Bioluminescence Imaging

Six-week-old female NOD.CB-17-Prkdcscid/J mice (NOD-SCID; Charles River Laboratories Japan, Inc., Kanagawa, Japan) were used for intracranial transplantation. A total of 1.0 × 10⁵ luc2-expressing breast cancer cell lines suspended in 4 µL D-PBS (−) (FUJIFILM Wako Pure Chemical Corporation) were injected intracranially using a 26-gauge syringe. After intracranial transplantation, 200 µL D-luciferin (15 mg/mL) (Gold Biotechnology, Inc., St. Louis, MO, USA) was injected intraperitoneally into NOD-SCID mice, and bioluminescence imaging was conducted every seven days using an in vivo imaging system (IVIS Lumina XRMS; Perkin-Elmer, Waltham, MA, USA). All animal experiments were approved by the Animal Committee of Waseda University.