Glu/Gal assay for mitochondrial toxicity

MC Michael J. Cox
SJ Steffen Jaensch
JW Jelle Van de Waeter
LC Laure Cougnaud
DS Daan Seynaeve
SB Soulaiman Benalla
SK Seong Joo Koo
IW Ilse Van Den Wyngaert
JN Jean-Marc Neefs
DM Dmitry Malkov
MB Mart Bittremieux
MS Margino Steemans
PP Pieter J. Peeters
JW Jörg Kurt Wegner
HC Hugo Ceulemans
EG Emmanuel Gustin
YC Yolanda T. Chong
HG Hinrich W. H. Göhlmann
request Request a Protocol
ask Ask a question
Favorite

The assay was conducted as previously described25 and used to assess the potential of test substances to trigger mitochondrial dysfunction. HepG2 cells cultured in glucose- or galactose-containing medium were exposed for 24 h to a concentration range (0–200 µM) of the test compounds. Following treatment, the cytotoxic potential (determined by ATP measurement) of each test compound was compared in glucose- versus galactose-culture conditions. A markedly higher cytotoxicity (IC50 Glu/Gal ratio > 5) in galactose-grown cells is indicative for mitochondrial dysfunction as a primary mode of action.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A