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MnSOD activity measurement

LL Lei Li
HH Hong-Hai Hong
SC Shi-Ping Chen
CM Cai-Qi Ma
HL Han-Yan Liu
YY Ya-Chao Yao
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MnSOD activity was measured with a commercial SOD kit (Cayman Chemical) according to the manufacturer’s protocol. Briefly, 1 mmol/L potassium cyanide was added in order to inhibit Cu/Zn-SOD and extracellular SOD, thus only MnSOD activity was detected. O2- was generated by adding hypoxanthine/xanthine oxidase and detected with tetrazolium salt through reading the absorbance at 450 nm.

Copyright and License information:  ©2016-05-07 The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.

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