The four-week-old male mice were subcutaneously injected with SW620R (2×106 cells in 200 μL volume). After 14 days of inoculation, the mice were treated with either vehicle (control), 5-FU (30mg/kg*3/week, ip), or the combination of VP, AG1478, and 5-FU (VP 50mg/kg*3/week, AG1478 50mg/kg*3/ week, 5-FU 30mg/kg*3/ week, ip) for 2 weeks. Then, the mice were sacrificed, and the tumors were collected and weighted.
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