3.1. Materials

Preparation of the starting leukotriene A4 hydrolase structure was performed using Discovery Studio (DS) 2017 from Biovia^® (formerlyAccelrys^®) Software Inc. (San Diego, CA, USA) [45]. Pharmacophore modeling and virtual screening were performed using DS. Docking of proposed inhibitors was performed using CDOCKER within DS [46]. Presentation quality images was generated using DS. Leukotriene A4 Hydrolase (human recombinant), LTA4 methyl ester, LTB4 ELISA kit and Ultra-Pure water were purchased from Cayman Chemical (Ann Arbor, MI, USA). The selected compounds were purchased from Maybridge Chemical Holdings Ltd., UK (which is a brand of Thermo Fisher Scientific, Waltham, MA, USA) via local vendors.