Alizarin red S (ARS) staining and quantification

YT Yiman Tang
XZ Xiao Zhang
WG Wenshu Ge
YZ Yongsheng Zhou
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After osteogenic induction for 14 days, cells were subjected to matrix mineralization as previously described [23]. Briefly, the cells were fixed for 30 min in 95% ethanol before being stained for 10 min with Alizarin red S (0.1%, pH 4.2). Calcium deposition was assessed quantitatively by eluting ARS with 100 mM cetylpyridinium chloride for 1 h and then detected by spectrophotometric absorbance at 562 nm.

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