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The substrate was modeled as a rigid plane, subdivided into right isosceles triangles with area of 2.102 μm2. Triangles on the plane were used to quantify traction by summing force exerted by the cell on each triangle with a defined area; this way, traction maps were generated (details in section Output metric calculation). Stiffness perceived by the cell arose from springs used to represent ligand molecules with a specific stiffness (kECM) as part of FAs and discussed in section Adhesion.
Copyright and License information: ©2020 Vargas, Gonçalves, Heck, Smeets, Lafuente-Gracia, Ramon and Van Oosterwyck.
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