Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

crRNA and sequence primer design

JH Julian J. Freen‐van Heeren
BP Branka Popović
AG Aurélie Guislain
MW Monika C. Wolkers
request Request a Protocol
ask Ask a question
Favorite

crRNAs and sequence primers were designed using the CRISPR and Primer design tools in Benchling (https://benchling.com, Table 1). Sequences were verified to be specific for the target of interest via BlastN and PrimerBlast (both NCBI).

Primers used in this study

Copyright and License information:  ©2020 The Authors. published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A