3.3. Minimum Inhibitory Concentration (MIC)

The broth microdilution method was utilized to determine MIC values of coumarin-substituted pyrazole derivatives against different clinically important S. aureus strains, B. subtilis and S. epidermidis according to the guidelines outlined by the Clinical and Laboratory Standards Institute (CLSI) as reported in our recent papers [9]. The starting concentration of compounds for MIC determination was 50 μg/mL serially diluted down the wells and the MIC values were recorded in duplicates in three independent experiments on different days.