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2.2. Chemicals and Reagents

MS Mussie Sium
PK Patrick Kareru
JK Joseph Keriko
BG Berhane Girmay
GM Ghebrehiwet Medhanie
SD Semere Debretsion
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Analytical grade chemicals and reagents were purchased from Sigma-Aldrich Company. 65% nitric acid (HNO3), 30% hydrogen peroxide (H2O2), 32% Hydro chloric acid (HCl), and 98% sulphuric acid (H2SO4) were used for digestion purposes. Ultrapure-deionized water (18 Ω) was used throughout the study. The glassware was soaked in 3 M HNO3 for the whole night and washed and rinsed with deionized water to minimize the chances of interferences. All the chemical analyses were conducted under extractor hood and a digital IR Vortex Mixer (S/N296058 made in Italy) was used for mixing of the solutions.

Copyright and License information:  ©2016 Mussie Sium et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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