4.6. Nitric Oxide Assay

Griess reaction was carried out to measure nitric oxide concentration in cell culture medium. In brief, culture medium of cell treated in presence or absence of TNF-α, followed by incubation with adenine was collected and mixed with Griess reagent in a ratio of 1:1 and absorbance at 540 nm was measured. The concentration of nitrite was measured using sodium nitrite (NaNO₂) as a standard.