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2.5. Western blot analysis of mitochondrial isolates
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Protein quantification was performed using a Pierce bicinchoninic acid protein assay kit (ThermoFisher), with electrophoresis using SDS-PAGE and immunodetection using calnexin (Abcam, ab22595) and ATP5A (Abcam, ab14748) followed by fluorescent DyLight secondary antibodies (Invitrogen, SA5-10036 and 35519) and visualisation using a CLx imaging system scanner (LI-COR Bioscience), using Geneflow BLUeye protein ladder (245kDa) ladder (Supplementary Fig. 1).
Copyright and License information: ©2020 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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