2.9. Platelet Adhesion Assay

Polymer sample films were trimmed to a size of 1 cm × 1 cm and placed into a 24-well plate sterilized with 75% ethanol 8 times on the orbital shaker at 100 rpm. Then, samples were immersed in a 10 mL Hepes-Tyrodes buffer solution for 1 h. Then, the buffer solution was removed and 10 mL of platelet-rich plasma was added into each well for incubation under 37 °C and 5% CO2 condition for 1 h. Then, platelet-rich plasma was replaced by 10 mL Hepes-Tyrodes buffer solution under 50 rpm shaking for 1 min 3 times. Then, the sample films were dehydrated by rinsing with 0%, 25%, 50%, 75%, and 100% ethanol subsequently for 1 min each, and later followed by complete dehydration with HMDS immersion for 5 min. Finally, HMDS was removed and dried before the sputter-coating with Au for the SEM examination.