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Nude Mouse Tumor Xenograft Model

WS Weixuan Sun
WN Wenting Nie
ZW Zhaoyi Wang
HZ Haolong Zhang
YL Yezhou Li
XF Xuedong Fang
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Male athymic BALB/c nude mice were purchased from the National Laboratory Animal Center (Beijing, China). Each mouse was injected subcutaneously with HCT-116 cells to establish a mouse xenograft model. A total of 5 × 106 shRNAHAGLR-HCT-116 and NC-HCT-116 cells were suspended in 100 μL phosphate buffer and injected subcutaneously into nude mice. Five weeks later, mice were euthanized. The study has been approved by the Ethics Committee of Beijing Friendship Hospital and is based on the the National Institutes of Health’s Guide to the Care and Use of Laboratory Animals.

Copyright and License information:  ©2020 Sun et al.
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