Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Measurement of plasma TNF-α, IL-6 and hs-CRP levels

DO Delicia Shu Qin Ooi
SO Sze Min Ong
ME Ming Hui Eng
YC Yiong Huak Chan
YL Yung Seng Lee
AL Adrian Fatt Hoe Low
MC Mark Yan-Yee Chan
CH Chew-Kiat Heng
request Request a Protocol
ask Ask a question
Favorite

Plasma tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured using ELISA kits for human TNF-α (ThermoFisher Scientific) and human IL-6 (Abcam). Plasma high sensitivity-C reactive protein (hs-CRP) levels were measured by the hs-CRP immunoturbidimetric assay on a Beckman Coulter Olympus AU5800 analyzer. All assays were carried out in accordance with the manufacturers’ protocol and each plasma sample was measured in duplicates.

Copyright and License information:  ©2020 Ooi et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A