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The analysis of SOD (EC 1.15.1.1) activity is based on the ability of SOD to inhibit the process of epinephrine self-oxidation in an alkaline medium according to the method of Misra and Firidovich [85]. In the reaction of colored adrenochrome formation, the superoxide anion radical is formed as an intermediate product. SOD activity was measured by monitoring the increase of absorbance at 480 nm and calculated in U/g hemoglobin (Hb) [83].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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