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A total of 5 × 103 MCF7 and MCF7-TAM12.5 cells were seeded onto six-well plates. MCF7-TAM12.5 cells were treated with or without 12.5 μM tamoxifen and allowed to grow for 1 week until the colonies could be observed. The colonies were fixed with 4% paraformaldehyde (Sigma-Aldrich, cat# P6158) solution for 15 min and subsequently stained with 0.2% crystal violet solution (Riedel-de Haën, Munich, Bayern, Germany, cat# 32675). The stained colonies were imaged with an inverted microscope (Nikon E400, Tokyo, Japan).
Copyright and License information: ©2020 Chang, Cheung and Kuo.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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