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Cell apoptosis was determined by the TUNEL assay performed using a TUNEL kit (C1098, Beyotime, Shanghai, China) following manufacturer’s instructions. Processed samples were incubated in a TUNEL reaction mixture (50 μL) for 60 min at 37 °C under dark conditions. Color developed in DAB coloring solution. Brown is apoptotic cells positive for TUNEL staining. Apoptosis index in the cortex area was measured using this formula: apoptosis index (%) = (apoptotic neurons/ total neurons) X 100%.
Copyright and License information: ©2020 Zhao et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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