Mice were anesthetized by 1% pentobarbital sodium (50 mg/kg, i.p.) for micro-CT scans with a GE eXplore Locus (GE Healthcare). All scans were performed in the same mode: 80 kV X-ray voltage, 450 μA current, 0.045 mm effective pixel size, 360° tomographic rotation and a rotation step of 0.9° with an exposure time of 400 ms for each view. All scans were reconstructed as 3D images by eXplore MicroView 2.0 analysis software with the same parameters.
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