Western blotting

Equal amounts of protein were homogenized and solubilized in 2x SDS-sample buffer, separated on 4 to 12% SDS-polyacrylamide gels, transferred to Immobilon-P and immunoblotted. Gels and blots were scanned on a Bio-Rad ChemiDoc MP imaging system. Protein levels were normalized to total protein using Bio-Rad Stain-Free imaging technology. An unpaired two-tailed t test was performed to compare between two groups. All statistical analysis was conducted using Prism software. The raw Western blots are shown in the Supplementary Figures.

Unless otherwise indicated, antibodies were from Cell Signaling Technologies (CS) or AbCAM (Ab) and used at a 1 to 2000 dilution. The molecular weights of the proteins shown in the Western blots are also included along with the catalog numbers: Tau, CS4019, 50–80 kDa; Amyloid Beta, 6E10, Signet9320; ApoE (pan), CS13366, 35 kDa; PGAM, CS11896, 28 kDa; UCHL1, CS11896, 27 kDa; KU70, CS4588, 70 kDa; ADCY1, ABclonalA9760, 100 kDa; CAMK2 (pan), CS4436, 50 kDa; FASN, CS3189, 273 kDa; GPI, CS57893S, 60 kDa; CKB, Ab92452, 43 kDa; LRP, CS64099, 85 kDa; HSP90β, CS7411S, 90 kDa; HSP75, CS95345S, 75 kDa; HSP70, CS9965, 70 kDa; HSP40, CS9965, 40 kDa; Coronin, CS60615, 60 kDa; Drebrin, CS5052, 120 kDa; Tubulin, CS2148, 52 kDa; Gelsolin, CS12953, 83 kDa; Dynamin 1, CS4565, 100 kDa; 14–3-3, CS8312, 27 kDa; Ub, CS3936; HNE, Cell Biolabs STA-034; MG, Cell Biolabs STA-011; CML, Ab27684; AGE, Millipore 9890; Acetyl-lysine, CS9814.