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Locomotor Activity Assay

ES Ekta J. Shah
KG Katherine Gurdziel
DR Douglas M. Ruden
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To measure locomotor activity, individual flies (24 biological replicates/condition) for control and TBI condition were placed in tubes containing regular fly food in a Drosophila activity monitoring system which measures the number of times a given fly crosses an infrared beam (TriKinetics Inc., Waltham, MA) (44). The activity was assessed for 2 days. Flies were subjected to 12-hr light/dark cycles with activity summarized every 30 min producing 96 timepoints of data. The number of beam breaks occurring as a result of fly movement in 30-min time-bins before the specified time-point are plotted as locomotor activity for that time-point. Flies that did not live through the recording period were not used in the calculations. Repeated measures ANOVA with Fisher's Least Significant Difference (LSD) and Bonferroni for multiple comparisons test was used to compute statistical significance (p < 0.05) between control and TBI groups using SPSS.

Copyright and License information:  ©2020 Shah, Gurdziel and Ruden.
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