General

The Kansas State University Institutional Animal Care and Use Committee approved the protocols used for these studies. Four groups of pigs were used in two experiments with groups 1 and 2 combined to represent experiment 1, and groups 3 and 4 combined to represent experiment 2. Groups 1 and 3 pigs were housed at the Kansas State University Segregated Early Weaning Facility in Manhattan, KS. Group 2 pigs were housed in a commercial research facility in southwestern Minnesota. Group 4 pigs were housed at the Kansas State University Swine Teaching and Research Center in Manhattan, KS. The research facilities were environmentally controlled. For groups 1 and 3, each pen (1.2 × 1.2 m) had tri-bar flooring and contained one 4-hole dry self-feeder and one cup waterer to provide ad libitum access to feed and water. For group 2, each pen (3.7 × 2.3 m) had plastic slatted flooring and contained one 6-hole dry self-feeder and one pan waterer to provide ad libitum access to feed and water. For group 4, each pen (1.2 × 1.5 m) had tri-bar flooring and contained one 4-hole dry self-feeder and one nipple waterer to provide ad libitum access to feed and water. Dietary treatments for groups 1, 3, and 4 were manufactured at the O.H. Kruse Feed Mill in Manhattan, KS. Group 2 dietary treatments were manufactured in a commercial feed mill located in Pipestone, MN.