EC109 cells (1 × 106 cells/mL) were treated with ART and/or OXA for up to 48 hours. For cell cycle analysis, cells were harvested, fixed with 70% cold ethanol, incubated with RNase, and stained with propidium iodide. The cell cycle was detected using flow cytometry (BD Biosciences) and data was analyzed using FlowJo v7.6 software (version 3.2, Verity Software House, USA).
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