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Homogeneous time-resolved fluorescence binding assay

MG Minmin Ge
ZH Zhengping Hu
XC Xiao Chen
GD Guangying Du
HW Hongbo Wang
RL Rumeng Liu
LY Liang Ye
JT Jingwei Tian
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Using a PD-1/PD-L1 binding assay kit (Cisbio, Bedford, MA, USA), the binding effect was determined in accordance with the manufacturer’s instructions. BMS936559 was used as the positive control. The homogeneous time-resolved fluorescence binding (HTRF) ratio was calculated using the following formula: HTRF ratio = (A665nm/A620nm) × 104.

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