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In Vitro Kinase Assay

RS Randy S. Schrecengost
CG Cecelia L. Green
YZ Yan Zhuang
SK Staci N. Keller
RS Ryan A. Smith
LM Lynn W. Maines
CS Charles D. Smith
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ABC1183 was screened by ThermoFisher Scientific SelectScreen services (Waltham, MA) at a single 10-μM dose against a panel of 414 human kinases using ATP at Km [app]. All kinases were screened in duplicate and data were confirmed to meet stringent quality control standards for reproducibility. Kinases inhibited by greater than 60% were further confirmed through dose response IC50 assays, carried out by ThermoFisher Scientific.

Copyright and License information:  ©2018 by The American Society for Pharmacology and Experimental Therapeutics

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