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Quantitative analysis of 5,7-dimethoxyflavone by thin-layer chromatography image analysis
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The 5,7-dimethoxyflavone spots on the developed TLC plates were photographed under short wave UV (254 nm) by a digital camera. Peak area of each spot was quantitated using ImageJ free software (Department of Health and Human Services, National Institutes of Health (NIH) in the United State). The content of 5,7-dimethoxyflavone was determined by comparing peak area to the calibration curve obtained from the same TLC plate.
Copyright and License information: ©2018 Journal of Advanced Pharmaceutical Technology & Research
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