Acute oral toxicity

Acute oral toxicity of all the selected plant extracts was performed as per OECD guidelines 423. A limit test at 2000 mg/kg body weight of the extracts was administered. Briefly, two thousand milligrams of the test substance per kilogram of body weight were administered to 3 healthy mice by oral gavages. The animals were observed for mortality, signs of gross toxicity, and behavioral changes at least once daily for 14 days. Body weights were recorded before administration and again on days 7 and 14 (day of termination). Necropsies were performed on all animals at terminal sacrifice (Ethics committee approval number: JSSCP/IAEC/PH. D/PH. COLOGY/02/2014-15).