Induction of experimental diabetic neuropathy

Experimental rats were fasted overnight and injected with STZ at a multiple dose of 40 mg/kg body weight for 3 consecutive days. The solution was injected intraperitoneally within 5 min after dissolving in citrate buffer pH 4.5.[9] The rats in Group NC were injected with distilled water as a vehicle control. The animals were allowed to drink 5% glucose solution overnight to overcome the drug-induced hypoglycemia. Random blood glucose was estimated at the time of induction of diabetes and was checked regularly until stable hyperglycemia was achieved. After a week time for the development of diabetes, the mice with moderate diabetes having glycosuria and hyperglycemia (blood glucose levels of 250 mg/dl) were included in the study as stable hyperglycemic animals.