Antimicrobial screening

The antibacterial and antifungal activities were tested by agar well diffusion method[15] using Mueller-Hinton agar media and sterilized potato dextrose agar media, respectively. The antimicrobial activity of synthesized compounds was tested against several microorganisms: Gram (+)ve bacteria (Staphylococcus aureus and Bacillus subtilis), Gram (-)ve bacteria (Escherichia coli and Pseudomonas aeruginosa), and Fungi (Aspergillus niger and Candida albicans). All test compounds were dissolved in DMSO and diluted to get final concentration 50 µg/mL. Under identical conditions, Streptomycin (50 µg/mL), and Fluconazole (50 µg/mL) were used as standard drug. DMSO was used as a control. Antimicrobial activity was determined by measuring the zone of inhibition.