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2.2. Determination of cell viability by MTT assay
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The BRL3A and HEK293 cells were seeded into 96-well plates at a cell density of 2 × 103 and 4 × 103 cells per well, respectively, and incubated for 24 h. Thereafter, these cells were treated with different concentrations of saikosaponin b2 (128.0, 64.0, 25.6, 12.8, 6.4, 1.28, and 0.128 μM) for 48 h. Subsequently, 20 μL MTT (50 g/L) was added to the cells, and incubated for 4 h. When formazan was formed, the culture medium was removed and 150 μL DMSO was added to dissolve the formazan. The optical density was measured at 570 nm.
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