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2.11. Short-Chain Fatty Acid (SCFA) and Lactic Acid Analysis
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High-performance liquid chromatography (HPLC) analysis was carried out using a Thermo Separation Products apparatus (Thermo Scientific, Waltham, MA, USA) equipped with RI Plus and PDA detectors and an Aminex HPX 87H + column (300 × 7.8 mm id) (Bio-Rad, Hercules, CA, USA). The flow rate was 0.6 mL/min, the temperature was 60 °C, and 0.005 M sulfuric acid was used as the phase. Identification and quantification of compounds was carried out based on standard compounds: formic acid, acetic acid, propionic acid, and lactic acid (Sigma) [19].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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