Cell viability assay

Cell proliferation was determined by MTT assay. The cells were seeded at 2 × 10⁵ cells/mL in 96 well plates and maintained for 48 h in a complete growth medium. They were then exposed to LA and OA (both 0, 10, 50, 100, and 250 µM) in a growth medium for 24 h and 48 h. The cells were then incubated with 5 mg/mL MTT for 4 h at 37^oC after which the formazan crystals were dissolved in DMSO. The absorbance of each well was measured at 490 nm by using a microplate reader (Multiskan GO, ThermoFisher Scientific, USA). The results are displayed as a percentage of untreated controls. Cell viability was calculated by the following formula: cell viability = (OD_treated – OD_blank)/(OD_control – OD_blank) wells × 100.