LDH assay

For LDH assay application, LDH cytotoxicity assay kit (Cayman Chemical Company^®, Ann Arbor, MI, USA) was used according to the manufacturer’s recommendations. The cells were transferred to 96-well plates and different concentrations of BN were applied for 72 h. To get rid of BN in the wells, 96-well plate was centrifuged at 400 g for 5 min. After that 100 µL supernatant and 100 µL of reaction mixture were transferred to a fresh 96-well plate and incubated for 30 min at room temperature. Finally, the absorbance of the cultures was analyzed at 490 nm using a microplate reader (Turkez et al. 2016).