Female BALB/c mice (5–6 weeks of age) were purchased from Orient Bio (Daejeon, Korea) and were used for experiments when they reached a body weight of 17–20 g (6–8 weeks of age). The mice were allowed to acclimate before experiments under specific pathogen-free conditions at the animal care facility of the College of Medicine (Inje University). Experimental procedures were reviewed and approved by the Institutional Animal Care and Use Committee of Inje University (protocol no. 2015-002).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.