4.1. Antibodies and Recombinant Selectins

LAM1-3 (anti-L-selectin) [50], PS5 (anti-PSGL-1) [35], WAPS 12.2 (anti-P-selectin, ATCC HB-299), H18/7 (anti-E-selectin, ATCC HB-11684), CSLEX-1 (anti-sLe^x; ATCC HB-10135), HECA-452 (anti-CLA; ATCC HB-11485), HERMES-1 (anti-CD44; DSHB University of Iowa, Iowa City, IA, USA) were purified from hybridoma culture medium. Fluorescent mAbs KPL-1 (anti-PSGL-1), DREG-56 (anti-L-selectin), HECA-452mAbs (anti-CLA), CD43, CD44 and isotype-matched controls were obtained from Becton Dickinson AG (Allschwil, Switzerland); PL1 (anti-PSGL-1) and 80H5 (anti-CD15) mAbs were from Beckman Coulter (Nyon, Switzerland). Phycoerythrin (PE)-conjugated goat anti-mouse Ig antibody and fluorescein isothiocyanate (FITC)-conjugated rabbit anti-human IgM antibody were from Dako (Agilent Technologies, Basel, Switzerland). Recombinant L-selectin was purchased from R&D Systems (Bio-Techne, Basel, Switzerland). PSGL-1-, P- and E-selectin/IgM heavy chain (µ) were produced as described [16,33,35].