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Total RNAs were extracted using the miRNeasy Mini Kit with a Qiacube (QIAGEN). Expression levels of miRNAs and messenger RNAs (mRNAs) were quantified using TaqMan MicroRNA Assays and Gene Expression Assays (Life Technologies), respectively. The expression of RNU6B and GAPDH was used as endogenous control for miRNA and mRNA data normalization, respectively. Specific primers were used for quantification (supplemental Table 1, available on the Blood Web site). The TaqMan Array Human Inflammation Panel (Life Technologies) was used according to the manufacturer‘s protocols, and relative gene expression was calculated using the comparative threshold cycle method.
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