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The culture medium was collected and analyzed for IL-6 and IL-8 using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s protocols (BD Biosciences 555220 and 555244, San Jose, CA, USA) with human recombinant IL-6 or IL-8 as the standard. IL-6 and IL-8 concentrations were calculated based on the standard curve and shown as pg/mL.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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