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4.13. Terminal Dexynucleotidyl Transferase-Mediated dUTP Nick End Labeling (TUNEL) Assay

LL Li Li
YY Yi Yang
MW Mingxia Wu
ZY Zanyang Yu
CW Chengqiang Wang
GD Guojun Dou
HH Hui He
HW Hongmei Wang
NY Na Yang
HQ Hongyi Qi
XX Xiaoyu Xu
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Apoptosis in tumor tissue was determined with a standard in situ TUNEL method following the manufacturer’s directions (Promega, Madison, WI, USA). Images of the sections were taken by a fluorescence microscope (Olympus, Tokyo, Japan) and images were analyzed by a person who was blinded to the animal group information. Apoptotic cells were counted as the number of TUNEL positive cells per mm2 of each examined section.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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