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2.7. Determination of Sorbitol Dehydrogenase (SDH) Activity
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The activity of SDH was measured from the reaction mixture contained: 0.7 mL of the NADH (355 μM, dissolved in 100 mM of the Tris buffer, pH = 6.6), 0.1 mL of the serum, and 0.2 mL of the fructose (2.5 M, dissolved in the Tris buffer, pH = 6.6) [18]. The changes in absorbance were recorded spectrophotometrically at 340 nm. The activity of SDH has been expressed in U/L.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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