2.7. PPARγ and PPARα Transcription Factor Assays

The DNA-binding activity of PPARγ or PPARα was assessed using PPARγ or PPARα Transcription Factor Assay Kits (Abcam), respectively, according to manufacturer’s instruction. Briefly, the nuclear proteins, extracted using a Nuclear Extraction Kit (Abcam), was added in wells immobilized with specific PPRE sequences. After incubation with the primary anti-PPARs antibody and HRP-conjugated secondary antibody subsequently, the absorbance was measured at 450 nm to determine the transcriptional activity of PPARγ or PPARα.