4.6. Gastric Lesion Induction by Ethanol

The induction of gastric lesion by ethanol was adapted from the method of Hollander and Tarnawski [58]. After 24 h of fasting, the rats (n = 7/group) were orally pre-treated at 1 h prior to induction. Group 1 (healthy) and 2 (gastric lesion control) received the vehicle (distilled water—10 mL/kg) orally. Group 3 received an oral dose of the 50 mg/kg ranitidine, and groups 4, 5 and 6 received an oral dose of the 125, 250 and 500 mg/kg respectively of K. brasiliensis leaf juice. Groups 7, 8 and 9 received an oral dose of the 125, 250 and 500 mg/kg respectively of K. pinnata leaf juice. After 1 h, all animals except Group 1 received an oral dose of the 0.5 mL/100 g absolute ethanol PA. The rats were then euthanized one hour later, the stomach removed by opening along the greatest curvature, washed with saline solution, and then macroscopically evaluated for measuring the injured areas. Stomach samples were stored at −80°C for analyses.