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4.5. DNA and Protein Interaction Confirmation in Yeast Cell

CL Chen Liu
XC Xin Chen
SZ Shengkui Zhang
CZ Changying Zeng
WW Wenquan Wang
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The full-length CDS and AP2-CDS (contains the AP2 domain from aa.67 to aa.166 in MeERF72 protein) of MeERF72 were inserted into the pGADT7 vector for the infusion prey protein, AD-MeERF72 and AD-AP2, respectively. A conserved ethylene responsive element (ERE) [30], TTTGAAAT (−1384 to −1376 from ATG), located in the promoter of MeSus1, and a bait strain, Y1HGold/pERE-AbA, was constructed with the sequence of 5′-AACTTTCCTCTCGTTGCTTGTTTGAAATTATCATTTTCTAGTAGAATA3′ (core sequence underline). Subsequently, pGADT7-MeERF72 and pGADT7-AP2 were transformed into two bait strains Y1HGold/pERE-AbA and Y1HGold/pSus1pro-AbA, respectively, and allowed to grow in SD/-Leu/AbA10 medium.

Copyright and License information:  ©2018 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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